Dai et al reported mMolecular cloning and characterization of a novel human hydroxysteroid
dehydrogenase-like 2 (HSDL2) cDNA from fetal brain.
Hydroxysteroid dehydrogenases (HSDs) are responsible for the biosynthesis of steroid hormones
and play a crucial role in mammalian physiology and development. By large-scale sequencing
analysis of a human fetal brain cDNA library, the authors isolated a novel human hydroxysteroid
dehydrogenase-like cDNA (HSDL2). This cDNA is 3211 bp in length, encoding a 418-amino-acid
polypeptide, which contains a typical motif for NAD(P)+-binding (TGxxxGxG), an SDR active site
motif (S-Y-K) and a sterol carrier protein domain. HSDL2 shows high similarity with the
homologues in the mouse and fruit fly. The HSDL2 gene is mapped to chromosome 9q32 and
contains 11 exons. RT-PCR analysis shows that the HSDL2 gene is widely expressed in human
tissues and the expression levels in liver, kidney, prostate, testis, and ovary are relatively high.