Double C2-like Domain-containing Protein, Alpha OKDB#: 2604
 Symbols: DOC2A Species: human
 Synonyms:  Locus:


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DNA Microarrays
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General Comment

NCBI Summary: There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2A is mainly expressed in brain and is suggested to be involved in Ca(2+)-dependent neurotransmitter release.
General function
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Cellular localization
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Ovarian function
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Expression regulated by
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Ovarian localization Oocyte
Comment Identification of oocyte-expressed genes abundant in fetal ovary Yao et al 2004 . The bovine oocyte microarray was used to identify genes highly expressed in fetal ovary (an enriched source of oocytes) relative to adult spleen and liver tissues. The goal of this experiment was to validate utility of the cDNA microarray and to identify additional oocyte-expressed genes abundant in fetal ovary relative to somatic tissues. Microarray experiments with fetal ovary vs. spleen revealed 24 clones with >2-fold higher expression in fetal ovary (P < 0.05). Likewise, microarray experiments comparing fetal ovary and liver RNA revealed 98 clones with >2-fold higher expression in fetal ovary (P < 0.05). Overall, 11 clones were found to be in common between the lists of genes expressed at higher levels (>2-fold) in fetal ovary than in either spleen or liver. These 11 clones were retrieved from the original source plates and subjected to DNA sequencing. Sequence analysis revealed that these 11 clones represent 7 different genes. Two clones encode for JY-1, a known bovine oocyte-specific gene (Yao J, Huang R, Kobayashi Y, Bettegowda A, Coussens PM, Ireland JJ, and Smith GW. Identification and characterization of expression of JY-1: a novel oocyte specific gene in cattle. Biol Reprod 68: 112, 2003.). Eight of the remaining nine clones encode for five previously identified genes (ribosomal protein L7a, dynein light chain, Doc2 , calmodulin, leucine-rich protein). The final clone encodes for a completely novel transcript (Table 3). Since a heterogeneous tissue source including both oocytes and somatic cells (fetal ovary) was used as an enriched source of bovine oocytes for microarray analysis, expression of above-described genes in isolated bovine oocytes (from adult ovaries) was confirmed by real-time RT-PCR analysis (Fig. 3).
Follicle stages
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Phenotypes
Mutations
Links
OMIM (Online Mendelian Inheritance in Man: an excellent source of general gene description and genetic information.)
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Beta
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created: 2004-09-22 06:25:43 by: Aaron J Hsueh, hsuehlab   email: aaron.hsueh@stanford.edu
home page: http://reprobio.stanford.edu/hsueh



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